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Attachment 7D - Impact Management Plan - EVNT Fauna species - Noosa Landfill Expansion
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Log in to see the plans as drawnAPPENDIX B — AMPHIBIAN HYGIENE PROTOCOL Technical manual Interim hygiene protocol for handling amphibians Disinfecting procedures Equipment such as wetsuits, waders, footwear, nets, buckets, vehicles and surgical equipment may act as camiers of disease, particularly if used in multiple sites. Household bleach (refer Table 1) is recommended for disinfection as it is widely available and is easy to dispose of under field conditions if proper precautions are followed (refer p4). There are other disinfectants as outlined in Table 1 that have been tested and shown to kill target pathogens. It is important to ensure that disinfectants do not leave any residue on equipment, as the residue can be harmful to frogs and their environment. Table 1: Disinfecting procedures Application | Disinfectant Concentration | Time Method Target Pathogen Surgical Benzalkonium chloride | 2mg/mi 1 min Immerse B.dendrobatidis equipment Ethanol 70% 1 min Immerse B.dendrobatidis Ranaviruses Collection Sodium hypochlorite" 4% 15min | Immerse Ranaviruses containers, (bleach) equipment, vehicles Sodium hypochlorite" 1% 1 min Immerse B. dendrobatidis (bleach) Didecyl dimethyl 1 in 500 dilution | 0.5 min | Immerse B. dendrobatidis ammonium chloride TriGene™ 1 in 5000 1 min Immerse B. dendrobatidis dilution Fio™ # 1 in 1500 1 min Immerse B. dendrobatidis dilution Virkon™ 2mgimi 1 min Immerse B. dendrobatidis Ranaviruses Complete drying >3 hr B. dendrobatidis Heat 60°C 30 min B. dendrobatidis Ranaviruses Heat 37°C 8 hr B. dendrobatidis Sterilising UV light 1 min Ranaviruses only Footwear Sodium hypochlorite" 1% 1 min Scrub/spray B. dendrobatidis (bleach) footwear Sodium hypochlorite" 4% 15min | Immerse Ranaviruses (bleach) footwear Didecyl dimethy! 1 in 500 dilution | 0.5 min | Scrub/spray B. dendrobatidis ammonium chloride footwear TriGene™ 1 in 5000 1 min Scrub B. dendrobatidis dilution footwear Fi0o™ # 1 in 1500 1 min Scrub B. dendrobatidis dilution footwear Complete drying >3 hr B. dendrobatidis Cloth (e.g. Hot wash 60°C or greater | 30 min B. dendrobatidis carry bags) Ranaviruses Page 6 of 9 - 130116
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